Biochemistry Course

(1) Acids and Bases. Definition. definition of pH and its interpretation. (2) Water and Solutions. Osmosis. Molarity, Molality, Normality. Buffer solution and their importance. pKa of buffer solution, (3) chemistry of carbohydrates: Definition, classification, Structural Isomerism, Optical isomerism. reactions. (4) chemistry of Proteins and Amino Acids: Definition, Structure and classification of Amino Acids. Essential amino acids. Definition, Structure of proteins, Functional classification of proteins. (5) chemistry of Lipids: Definition of lipids, classification of lipids, Phospholipids, Gangliosides, Cerebrosides, Glycolipids, Lipoproteins (definition, crassification and functions) Chemical reactions of Lipids. (6) Chemistry of Nucleic acids: Structure of DNA, RNA classification and structure of the various types of RNA. (7) Nutrition and Basal metabolism: BMI und- it, calculation, Specific dynamic action (sDA) , Nutritional requirements and their calculations. Protein energy malnutrition. (8) vitamins: definition, classification, Uses in the body and deficiency diseases. (9) Clinical biochemistry:(for MLT course only) (a) Photometry: Laws of photometry, absorbancg transmittance, Structure and components of a photometer. Types ofphotometry: colorimetry, spectrophotometry, flurometry. Choice of filters etc. (b) Electrophoresis principal