Short title, extent and commencement

(1) This Act may be called the Telangana Bio-fertilisers (Monitoring and Quality Control) Act, 2006. (2) It extends to the whole of the State of Telangana. (3) It shall be deemed to have come into force with effect on and from the 21st November, 2005. 1. APPARATUS: same as 1 A of Part B of Schedule II

2. REAGENTS: 2.1. Medium Use a plating medium of the following composition Malic acid 5.0 g Potassium hydroxide 4.0 g Di-potassium hydrogen phosphate 0.5 g Ferrous sulphate 0.05 g Manganese sulphate 0.01 g Magnesium sulphate 0.1 g Sodium chloride 0.2 g Calcium chloride 0.1 g Sodium Molybdate 0.002 g Distilled water 1000 ml Boromothymol blue 2.0 ml (0.5 % alcoholic solution ) Agar 1.7 g pH adjusted to 6.5 - 7.0

2.2. Sterlising & preparation procedure for plates: same as 2.3.1 and 2.3.2 of 1 A of Part B of Schedule-II

PREPARATION OF PLATING MEDIUM AND POURING Same as 2.3.3, 2.3.4, 2.3.5 & 2.3.6 of 1 A of Part B of Schedule – II

3. PREPARATION OF SERIAL DILUTIONS FOR PLATE COUNTS: same as 1 B of Part B of Schedule – II

4. Incubation of plates: 4.1. Label the plates and incubate at 28 +/- 30 C for 4 to 6 days. 4.2. Colony counting aids: Same as 4.2 of 1 A of Part B of Schedule-II